3R2V
Crystal structure of polymerase basic protein 2 E538-R753 from Influenza A virus A/Yokohama/2017/03 H3N2
Summary for 3R2V
| Entry DOI | 10.2210/pdb3r2v/pdb |
| Related | 2VY6 3KC6 3KHW 3L56 |
| Descriptor | PB2 C-terminal subunit, IODIDE ION, BORIC ACID, ... (4 entities in total) |
| Functional Keywords | structural genomics, seattle structural genomics center for infectious disease, ssgcid, influenza, flu, polymerase basic, pb2, h3n2, h1n1, h5n1, virus, viral protein, 2003 avian flu outbreak |
| Biological source | Influenza A virus |
| Total number of polymer chains | 1 |
| Total formula weight | 25700.63 |
| Authors | Seattle Structural Genomics Center for Infectious Disease (SSGCID) (deposition date: 2011-03-14, release date: 2011-03-30, Last modification date: 2023-09-13) |
| Primary citation | Smith, E.R.,Begley, D.W.,Anderson, V.,Raymond, A.C.,Haffner, T.E.,Robinson, J.I.,Edwards, T.E.,Duncan, N.,Gerdts, C.J.,Mixon, M.B.,Nollert, P.,Staker, B.L.,Stewart, L.J. The Protein Maker: an automated system for high-throughput parallel purification. Acta Crystallogr.,Sect.F, 67:1015-1021, 2011 Cited by PubMed Abstract: The Protein Maker is an automated purification system developed by Emerald BioSystems for high-throughput parallel purification of proteins and antibodies. This instrument allows multiple load, wash and elution buffers to be used in parallel along independent lines for up to 24 individual samples. To demonstrate its utility, its use in the purification of five recombinant PB2 C-terminal domains from various subtypes of the influenza A virus is described. Three of these constructs crystallized and one diffracted X-rays to sufficient resolution for structure determination and deposition in the Protein Data Bank. Methods for screening lysis buffers for a cytochrome P450 from a pathogenic fungus prior to upscaling expression and purification are also described. The Protein Maker has become a valuable asset within the Seattle Structural Genomics Center for Infectious Disease (SSGCID) and hence is a potentially valuable tool for a variety of high-throughput protein-purification applications. PubMed: 21904043DOI: 10.1107/S1744309111028776 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.3 Å) |
Structure validation
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