3R2V
Crystal structure of polymerase basic protein 2 E538-R753 from Influenza A virus A/Yokohama/2017/03 H3N2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-03-04 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97946 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 52.810, 106.540, 33.490 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.300 |
R-factor | 0.1816 |
Rwork | 0.180 |
R-free | 0.20940 |
Structure solution method | SAD, MOLECULAR REPLACEMENT |
Starting model (for MR) | 2vy6 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.278 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.330 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.040 | 0.319 |
Number of reflections | 46293 | 2983 |
<I/σ(I)> | 21.66 | 3.27 |
Completeness [%] | 97.6 | 86.7 |
Redundancy | 5.4 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 289 | InvaB.07055.c.D17 PD00118 at 24.51 mg/mL against 0.1 M MIB pH 6.0, 25% PEG 1500, 100 mM NaI, crystal tracking ID 220930b6, VAPOR DIFFUSION, SITTING DROP, temperature 289K |