3QV1
Crystal structure of the binary complex of photosyntetic A4 glyceraldehyde 3-phosphate dehydrogenase (GAPDH) with cp12-2, both from Arabidopsis thaliana.
Summary for 3QV1
| Entry DOI | 10.2210/pdb3qv1/pdb |
| Related | 3K2B |
| Descriptor | Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic, CP12 protein, NICOTINAMIDE-ADENINE-DINUCLEOTIDE, ... (7 entities in total) |
| Functional Keywords | rossmann fold, calvin cycle, nad, chloroplast, oxidoreductase-protein binding complex, oxidoreductase/protein binding |
| Biological source | Arabidopsis thaliana (mouse-ear cress,thale-cress) More |
| Cellular location | Plastid, chloroplast membrane; Peripheral membrane protein: P25856 Plastid, chloroplast : Q9LZP9 |
| Total number of polymer chains | 9 |
| Total formula weight | 249165.63 |
| Authors | Thumiger, A.,Fermani, S.,Falini, G.,Marri, L.,Sparla, F.,Trost, P. (deposition date: 2011-02-25, release date: 2012-02-29, Last modification date: 2024-11-06) |
| Primary citation | Fermani, S.,Trivelli, X.,Sparla, F.,Thumiger, A.,Calvaresi, M.,Marri, L.,Falini, G.,Zerbetto, F.,Trost, P. Conformational Selection and Folding-upon-binding of Intrinsically Disordered Protein CP12 Regulate Photosynthetic Enzymes Assembly. J.Biol.Chem., 287:21372-21383, 2012 Cited by PubMed Abstract: Carbon assimilation in plants is regulated by the reduction of specific protein disulfides by light and their re-oxidation in the dark. The redox switch CP12 is an intrinsically disordered protein that can form two disulfide bridges. In the dark oxidized CP12 forms an inactive supramolecular complex with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and phosphoribulokinase, two enzymes of the carbon assimilation cycle. Here we show that binding of CP12 to GAPDH, the first step of ternary complex formation, follows an integrated mechanism that combines conformational selection with induced folding steps. Initially, a CP12 conformation characterized by a circular structural motif including the C-terminal disulfide is selected by GAPDH. Subsequently, the induced folding of the flexible C-terminal tail of CP12 in the active site of GAPDH stabilizes the binary complex. Formation of several hydrogen bonds compensates the entropic cost of CP12 fixation and terminates the interaction mechanism that contributes to carbon assimilation control. PubMed: 22514274DOI: 10.1074/jbc.M112.350355 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
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