3QV1
Crystal structure of the binary complex of photosyntetic A4 glyceraldehyde 3-phosphate dehydrogenase (GAPDH) with cp12-2, both from Arabidopsis thaliana.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-09-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.931 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 142.670, 245.990, 138.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.200 - 2.000 |
| R-factor | 0.22776 |
| Rwork | 0.225 |
| R-free | 0.27243 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k2b |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.737 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.200 | 2.080 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.139 | 0.716 |
| Number of reflections | 163587 | |
| <I/σ(I)> | 5.9 | 1.3 |
| Completeness [%] | 99.2 | 98.2 |
| Redundancy | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 15% (w/v) PEG 4K, 0.6 M NaCl, 1 mM NAD and 0.1 M MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
