3QFJ

The complex between TCR A6 and human Class I MHC HLA-A2 with the modified TAX (Y5F) peptide

3QFJ の概要

• エントリーDOI: 10.2210/pdb3qfj/pdb
• 分子名称: HLA class I histocompatibility antigen, A-2 alpha chain, Beta-2-microglobulin, TAX(Y5F) peptide, ... (7 entities in total)
• 機能のキーワード: tax peptide, nonapeptide, mhc class i, hla-a2, tcr a6, cross-reactivity, y5f mutation, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P01892; Secreted: P61769
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 95250.40

構造登録者

Borbulevych, O.Y.,Baker, B.M. (登録日: 2011-01-21, 公開日: 2012-01-11, 最終更新日: 2024-11-27)

主引用文献

Scott, D.R.,Borbulevych, O.Y.,Piepenbrink, K.H.,Corcelli, S.A.,Baker, B.M.
Disparate degrees of hypervariable loop flexibility control T-cell receptor cross-reactivity, specificity, and binding mechanism.
J.Mol.Biol., 414:385-400, 2011

PubMed Abstract: αβ T-cell receptors (TCRs) recognize multiple antigenic peptides bound and presented by major histocompatibility complex molecules. TCR cross-reactivity has been attributed in part to the flexibility of TCR complementarity-determining region (CDR) loops, yet there have been limited direct studies of loop dynamics to determine the extent of its role. Here we studied the flexibility of the binding loops of the αβ TCR A6 using crystallographic, spectroscopic, and computational methods. A significant role for flexibility in binding and cross-reactivity was indicated only for the CDR3α and CDR3β hypervariable loops. Examination of the energy landscapes of these two loops indicated that CDR3β possesses a broad, smooth energy landscape, leading to rapid sampling in the free TCR of a range of conformations compatible with different ligands. The landscape for CDR3α is more rugged, resulting in more limited conformational sampling that leads to specificity for a reduced set of peptides as well as the major histocompatibility complex protein. In addition to informing on the mechanisms of cross-reactivity and specificity, the energy landscapes of the two loops indicate a complex mechanism for TCR binding, incorporating elements of both conformational selection and induced fit in a manner that blends features of popular models for TCR recognition.

PubMed: 22019736
DOI: 10.1016/j.jmb.2011.10.006

実験手法

X-RAY DIFFRACTION (2.29 Å)