3QFJ
The complex between TCR A6 and human Class I MHC HLA-A2 with the modified TAX (Y5F) peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2010-08-19 |
Detector | MAR scanner 300 mm plate |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 224.008, 48.098, 92.959 |
Unit cell angles | 90.00, 91.02, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.290 |
R-factor | 0.221 |
Rwork | 0.218 |
R-free | 0.27100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2gj6 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.550 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.340 |
High resolution limit [Å] | 2.290 | 2.290 |
Rmerge | 0.066 | 0.598 |
Number of reflections | 44526 | |
<I/σ(I)> | 25.5 | |
Completeness [%] | 99.1 | 98.5 |
Redundancy | 5.7 | 5.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 297 | PEG 24%, TRIS 0.1 M, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |