3Q23

X-ray crystal structure of the N4 mini-VRNAP and P2_7a promoter transcription initiation complex with GMPCPP and Manganese: sustrate complex II

3Q23 の概要

• エントリーDOI: 10.2210/pdb3q23/pdb
• 関連するPDBエントリー: 3Q0A 3Q22 3Q24
• 分子名称: Virion RNA polymerase, DNA (5'-D(*TP*GP*CP*CP*TP*CP*CP*CP*AP*GP*GP*CP*AP*TP*CP*CP*AP*AP*AP*AP*GP*AP*AP*GP*CP*GP*GP*AP*GP*CP*TP*TP*CP*TP*TP*C)-3'), PHOSPHOMETHYLPHOSPHONIC ACID GUANYLATE ESTER, ... (6 entities in total)
• 機能のキーワード: protein-dna complex, two-metal catalysis, gmpcpp, gpcpp, de novo transcription initiation, nucleotidyltransferase, initiation complex, dna-hairpin, virion rna polymerase, phosphate ion, rna polymerase, transferase-dna complex, transferase/dna
• 由来する生物種: Enterobacteria phage N4 (Bacteriophage N4)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 270899.74

構造登録者

Gleghorn, M.L.,Murakami, K.S. (登録日: 2010-12-19, 公開日: 2011-02-16, 最終更新日: 2024-02-21)

• 主引用文献: Gleghorn, M.L.,Davydova, E.K.,Basu, R.,Rothman-Denes, L.B.,Murakami, K.S.; X-ray crystal structures elucidate the nucleotidyl transfer reaction of transcript initiation using two nucleotides.; Proc.Natl.Acad.Sci.USA, 108:3566-3571, 2011; PubMed: 21321236; DOI: 10.1073/pnas.1016691108

実験手法

X-RAY DIFFRACTION (1.8 Å)