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3PZV

C2 crystal form of the endo-1,4-beta-glucanase from Bacillus subtilis 168

Summary for 3PZV
Entry DOI10.2210/pdb3pzv/pdb
Related3PZT 3PZU
DescriptorEndoglucanase (2 entities in total)
Functional Keywordsalpha/beta barrel, glycosyl hydrolase, cellulose binding, hydrolase
Biological sourceBacillus subtilis subsp. subtilis
Total number of polymer chains4
Total formula weight144592.81
Authors
Santos, C.R.,Paiva, J.H.,Akao, P.K.,Meza, A.N.,Silva, J.C.,Squina, F.M.,Ward, R.J.,Ruller, R.,Murakami, M.T. (deposition date: 2010-12-14, release date: 2011-09-14, Last modification date: 2024-02-21)
Primary citationSantos, C.R.,Paiva, J.H.,Sforca, M.L.,Neves, J.L.,Navarro, R.Z.,Cota, J.,Akao, P.K.,Hoffmam, Z.B.,Meza, A.N.,Smetana, J.H.,Nogueira, M.L.,Polikarpov, I.,Xavier-Neto, J.,Squina, F.M.,Ward, R.J.,Ruller, R.,Zeri, A.C.,Murakami, M.T.
Dissecting structure-function-stability relationships of a thermostable GH5-CBM3 cellulase from Bacillus subtilis 168.
Biochem.J., 441:95-104, 2012
Cited by
PubMed Abstract: Cellulases participate in a number of biological events, such as plant cell wall remodelling, nematode parasitism and microbial carbon uptake. Their ability to depolymerize crystalline cellulose is of great biotechnological interest for environmentally compatible production of fuels from lignocellulosic biomass. However, industrial use of cellulases is somewhat limited by both their low catalytic efficiency and stability. In the present study, we conducted a detailed functional and structural characterization of the thermostable BsCel5A (Bacillus subtilis cellulase 5A), which consists of a GH5 (glycoside hydrolase 5) catalytic domain fused to a CBM3 (family 3 carbohydrate-binding module). NMR structural analysis revealed that the Bacillus CBM3 represents a new subfamily, which lacks the classical calcium-binding motif, and variations in NMR frequencies in the presence of cellopentaose showed the importance of polar residues in the carbohydrate interaction. Together with the catalytic domain, the CBM3 forms a large planar surface for cellulose recognition, which conducts the substrate in a proper conformation to the active site and increases enzymatic efficiency. Notably, the manganese ion was demonstrated to have a hyper-stabilizing effect on BsCel5A, and by using deletion constructs and X-ray crystallography we determined that this effect maps to a negatively charged motif located at the opposite face of the catalytic site.
PubMed: 21880019
DOI: 10.1042/BJ20110869
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (2.867 Å)
Structure validation

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