3PZV
C2 crystal form of the endo-1,4-beta-glucanase from Bacillus subtilis 168
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-09-09 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.4586 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 155.359, 81.080, 114.270 |
Unit cell angles | 90.00, 104.29, 90.00 |
Refinement procedure
Resolution | 41.464 - 2.867 |
R-factor | 0.2335 |
Rwork | 0.230 |
R-free | 0.29610 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 1.261 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.500 | 3.000 |
High resolution limit [Å] | 2.867 | 2.900 |
Number of reflections | 30407 | |
Completeness [%] | 97.1 | 83.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 20% PEG3350 200 mM sodium Nitrate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |