3PGW

Crystal structure of human U1 snRNP

3PGW の概要

• エントリーDOI: 10.2210/pdb3pgw/pdb
• 分子名称: U1-A, U1 snRNA, DNA 5'-D(*AP*GP*GP*TP*AP*AP*GP*TP*A)-3', ... (11 entities in total)
• 機能のキーワード: protein-rna complex, u1 snrna, sm fold, sm core, rrm, splicing, mrna, snrnps, splicing factors, splicing-dna-rna complex, splicing/dna/rna
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Nucleus: P09012 P08621 Q66K91; Cytoplasm, cytosol : P62318 P62314 P62316 P62306 P62304
• タンパク質・核酸の鎖数: 22
• 化学式量合計: 464104.82

構造登録者

Weber, G.,Trowitzsch, S.,Kastner, B.,Luehrmann, R.,Wahl, M.C. (登録日: 2010-11-02, 公開日: 2010-12-29, 最終更新日: 2024-02-21)

主引用文献

Weber, G.,Trowitzsch, S.,Kastner, B.,Luhrmann, R.,Wahl, M.C.
Functional organization of the Sm core in the crystal structure of human U1 snRNP.
Embo J., 29:4172-4184, 2010

PubMed Abstract: U1 small nuclear ribonucleoprotein (snRNP) recognizes the 5'-splice site early during spliceosome assembly. It represents a prototype spliceosomal subunit containing a paradigmatic Sm core RNP. The crystal structure of human U1 snRNP obtained from natively purified material by in situ limited proteolysis at 4.4 Å resolution reveals how the seven Sm proteins, each recognize one nucleotide of the Sm site RNA using their Sm1 and Sm2 motifs. Proteins D1 and D2 guide the snRNA into and out of the Sm ring, and proteins F and E mediate a direct interaction between the Sm site termini. Terminal extensions of proteins D1, D2 and B/B', and extended internal loops in D2 and B/B' support a four-way RNA junction and a 3'-terminal stem-loop on opposite sides of the Sm core RNP, respectively. On a higher organizational level, the core RNP presents multiple attachment sites for the U1-specific 70K protein. The intricate, multi-layered interplay of proteins and RNA rationalizes the hierarchical assembly of U snRNPs in vitro and in vivo.

PubMed: 21113136
DOI: 10.1038/emboj.2010.295

実験手法

X-RAY DIFFRACTION (4.4 Å)