3OQ9

Structure of the FAS/FADD death domain assembly

3OQ9 の概要

• エントリーDOI: 10.2210/pdb3oq9/pdb
• 分子名称: Tumor necrosis factor receptor superfamily member 6, Protein FADD (2 entities in total)
• 機能のキーワード: apoptosis, disc, fas, fadd
• 由来する生物種: Mus musculus (mouse); 詳細
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P25446
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 109239.28

構造登録者

Kabaleeswaran, V.,Wu, H. (登録日: 2010-09-02, 公開日: 2010-10-13, 最終更新日: 2024-02-21)

主引用文献

Wang, L.,Yang, J.K.,Kabaleeswaran, V.,Rice, A.J.,Cruz, A.C.,Park, A.Y.,Yin, Q.,Damko, E.,Jang, S.B.,Raunser, S.,Robinson, C.V.,Siegel, R.M.,Walz, T.,Wu, H.
The Fas-FADD death domain complex structure reveals the basis of DISC assembly and disease mutations.
Nat.Struct.Mol.Biol., 17:1324-1329, 2010

PubMed Abstract: The death-inducing signaling complex (DISC) formed by the death receptor Fas, the adaptor protein FADD and caspase-8 mediates the extrinsic apoptotic program. Mutations in Fas that disrupt the DISC cause autoimmune lymphoproliferative syndrome (ALPS). Here we show that the Fas-FADD death domain (DD) complex forms an asymmetric oligomeric structure composed of 5-7 Fas DD and 5 FADD DD, whose interfaces harbor ALPS-associated mutations. Structure-based mutations disrupt the Fas-FADD interaction in vitro and in living cells; the severity of a mutation correlates with the number of occurrences of a particular interaction in the structure. The highly oligomeric structure explains the requirement for hexameric or membrane-bound FasL in Fas signaling. It also predicts strong dominant negative effects from Fas mutations, which are confirmed by signaling assays. The structure optimally positions the FADD death effector domain (DED) to interact with the caspase-8 DED for caspase recruitment and higher-order aggregation.

PubMed: 20935634
DOI: 10.1038/nsmb.1920

実験手法

X-RAY DIFFRACTION (6.8 Å)