3OOR
I-SceI mutant (K86R/G100T)complexed with C/G+4 DNA substrate
Summary for 3OOR
Entry DOI | 10.2210/pdb3oor/pdb |
Related | 1R7M 3C0W 3C0X 3OOL |
Descriptor | Intron encoded endonuclease I-SceI, 5'-D(*CP*AP*CP*GP*CP*TP*AP*GP*GP*GP*AP*TP*AP*AP*CP*CP*GP*GP*GP*TP*AP*AP*TP*AP*C)-3', 5'-D(*GP*GP*TP*AP*TP*TP*AP*CP*CP*CP*GP*GP*TP*TP*AP*TP*CP*CP*CP*TP*AP*GP*CP*GP*T)-3', ... (5 entities in total) |
Functional Keywords | homing endonuclease, intron homing, laglidadg, hydrolase-dna complex, i-scei mutant (k86r/g100t), hydrolase/dna |
Biological source | Saccharomyces cerevisiae (yeast) |
Cellular location | Mitochondrion: P03882 |
Total number of polymer chains | 3 |
Total formula weight | 43536.78 |
Authors | Joshi, R.,Chen, J.-H.,Golden, B.L.,Gimble, F.S. (deposition date: 2010-08-31, release date: 2010-11-17, Last modification date: 2023-09-06) |
Primary citation | Joshi, R.,Ho, K.K.,Tenney, K.,Chen, J.H.,Golden, B.L.,Gimble, F.S. Evolution of I-SceI Homing Endonucleases with Increased DNA Recognition Site Specificity. J.Mol.Biol., 405:185-200, 2011 Cited by PubMed: 21029741DOI: 10.1016/j.jmb.2010.10.029 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.5 Å) |
Structure validation
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