3OOR
I-SceI mutant (K86R/G100T)complexed with C/G+4 DNA substrate
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A | Intron encoded endonuclease I-SceI | polymer | 237 | 28058.6 | 1 | UniProt (P03882) Pfam (PF03161) In PDB | Saccharomyces cerevisiae (yeast) | 21S rRNA intron maturase, Homing endonuclease omega |
2 | C | 5'-D(*CP*AP*CP*GP*CP*TP*AP*GP*GP*GP*AP*TP*AP*AP*CP*CP*GP*GP*GP*TP*AP*AP*TP*AP*C)-3' | polymer | 25 | 7717.0 | 1 | |||
3 | D | 5'-D(*GP*GP*TP*AP*TP*TP*AP*CP*CP*CP*GP*GP*TP*TP*AP*TP*CP*CP*CP*TP*AP*GP*CP*GP*T)-3' | polymer | 25 | 7640.9 | 1 | |||
4 | A, D | CALCIUM ION | non-polymer | 40.1 | 3 | Chemie (CA) | |||
5 | water | water | 18.0 | 11 | Chemie (HOH) |
Sequence modifications
A: 1 - 235 (UniProt: P03882)
PDB | External Database | Details |
---|---|---|
Arg 86 | Lys 86 | engineered mutation |
Thr 100 | Gly 100 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 3 |
Total formula weight | 43416.5 | |
Non-Polymers* | Number of molecules | 3 |
Total formula weight | 120.2 | |
All* | Total formula weight | 43536.8 |