1R7M

The homing endonuclease I-SceI bound to its DNA recognition region

Summary for 1R7M

• Entry DOI: 10.2210/pdb1r7m/pdb
• Descriptor: 5'-D(*CP*AP*CP*GP*CP*TP*AP*GP*GP*GP*AP*TP*AP*AP*CP*AP*GP*GP*GP*TP*AP*AP*TP*AP*C)-3', 5'-D(*GP*GP*TP*AP*TP*TP*AP*CP*CP*CP*TP*GP*TP*TP*AP*TP*CP*CP*CP*TP*AP*GP*CP*GP*T)-3', Intron-encoded endonuclease I-SceI, ... (5 entities in total)
• Functional Keywords: protein-dna complex, endonuclease, laglidadg, beta-saddle, hydrolase-dna complex, hydrolase/dna
• Biological source: Saccharomyces cerevisiae (baker's yeast)
• Cellular location: Mitochondrion: P03882
• Total number of polymer chains: 6
• Total formula weight: 86388.78

Authors

Moure, C.M.,Gimble, F.S.,Quiocho, F.A. (deposition date: 2003-10-21, release date: 2004-10-26, Last modification date: 2024-02-14)

• Primary citation: Moure, C.M.,Gimble, F.S.,Quiocho, F.A.; The crystal structure of the gene targeting homing endonuclease I-SceI reveals the origins of its target site specificity; J.Mol.Biol., 334:685-695, 2003; PubMed: 14636596; DOI: 10.1016/j.jmb.2003.09.068

Experimental method

X-RAY DIFFRACTION (2.25 Å)