1R7M
The homing endonuclease I-SceI bound to its DNA recognition region
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2001-03-19 |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 80.600, 90.700, 115.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 24.350 - 2.250 |
R-factor | 0.235 |
Rwork | 0.235 |
R-free | 0.26700 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.800 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.330 |
High resolution limit [Å] | 2.250 | 2.250 |
Number of reflections | 36883 | |
<I/σ(I)> | 18.1 | 18.1 |
Completeness [%] | 91.7 | 91.7 |
Redundancy | 3.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.7 | 277 | PEG400, sodium acetate, calcium chloride, postassium chloride, pH 4.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG400 | ||
2 | 1 | 1 | sodium acetate | ||
3 | 1 | 1 | calcium chloride | ||
4 | 1 | 1 | postassium chloride | ||
5 | 1 | 1 | H2O | ||
6 | 1 | 2 | PEG400 | ||
7 | 1 | 2 | calcium chloride | ||
8 | 1 | 2 | H2O |