3OGO

Structure of the GFP:GFP-nanobody complex at 2.8 A resolution in spacegroup P21212

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3OGO の概要

• エントリーDOI: 10.2210/pdb3ogo/pdb
• 分子名称: Green fluorescent protein, GFP-nanobody, ISOPROPYL ALCOHOL, ... (4 entities in total)
• 機能のキーワード: gfp, gfp-nanobody, beta-barrel, antibody, luminescent protein-immune system complex, fluorescent protein-immune system complex, fluorescent protein/immune system
• 由来する生物種: Aequorea victoria (Jellyfish); 詳細
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 167800.02

構造登録者

Kubala, M.H.,Kovtun, O.,Alexandrov, K.,Collins, B.M. (登録日: 2010-08-17, 公開日: 2010-08-25, 最終更新日: 2026-03-18)

主引用文献

Kubala, M.H.,Kovtun, O.,Alexandrov, K.,Collins, B.M.
Structural and thermodynamic analysis of the GFP:GFP-nanobody complex.
Protein Sci., 19:2389-2401, 2010

PubMed Abstract: The green fluorescent protein (GFP)-nanobody is a single-chain VHH antibody domain developed with specific binding activity against GFP and is emerging as a powerful tool for isolation and cellular engineering of fluorescent protein fusions in many different fields of biological research. Using X-ray crystallography and isothermal titration calorimetry, we determine the molecular details of GFP:GFP-nanobody complex formation and explain the basis of high affinity and at the same time high specificity of protein binding. Although the GFP-nanobody can also bind YFP, it cannot bind the closely related CFP or other fluorescent proteins from the mFruit series. CFP differs from GFP only within the central chromophore and at one surface amino acid position, which lies in the binding interface. Using this information, we have engineered a CFP variant (I146N) that is also able to bind the GFP-nanobody with high affinity, thus extending the toolbox of genetically encoded fluorescent probes that can be isolated using the GFP-nanobody.

PubMed: 20945358
DOI: 10.1002/pro.519

実験手法

X-RAY DIFFRACTION (2.8 Å)