3OG8

Crystal structure of human RIG-I CTD bound to a 14-bp blunt-ended dsRNA

Summary for 3OG8

• Entry DOI: 10.2210/pdb3og8/pdb
• Related: 3LRN 3LRR
• Descriptor: Antiviral innate immune response receptor RIG-I, RNA (5'-R(*GP*GP*CP*GP*CP*GP*CP*GP*CP*GP*CP*GP*CP*C)-3'), ZINC ION, ... (4 entities in total)
• Functional Keywords: innate immunity, viral rna sensing, rna binding domain, ips-1, cytosolic, hydrolase-rna complex, hydrolase/rna
• Biological source: Homo sapiens (human); More
• Cellular location: Cytoplasm: O95786
• Total number of polymer chains: 4
• Total formula weight: 39239.28

Authors

Li, P. (deposition date: 2010-08-16, release date: 2010-11-03, Last modification date: 2024-02-21)

Primary citation

Lu, C.,Ranjith-Kumar, C.T.,Hao, L.,Kao, C.C.,Li, P.
Crystal structure of RIG-I C-terminal domain bound to blunt-ended double-strand RNA without 5' triphosphate.
Nucleic Acids Res., 39:1565-1575, 2011

PubMed Abstract: RIG-I recognizes molecular patterns in viral RNA to regulate the induction of type I interferons. The C-terminal domain (CTD) of RIG-I exhibits high affinity for 5' triphosphate (ppp) dsRNA as well as blunt-ended dsRNA. Structures of RIG-I CTD bound to 5'-ppp dsRNA showed that RIG-I recognizes the termini of dsRNA and interacts with the ppp through electrostatic interactions. However, the structural basis for the recognition of non-phosphorylated dsRNA by RIG-I is not fully understood. Here, we show that RIG-I CTD binds blunt-ended dsRNA in a different orientation compared to 5' ppp dsRNA and interacts with both strands of the dsRNA. Overlapping sets of residues are involved in the recognition of blunt-ended dsRNA and 5' ppp dsRNA. Mutations at the RNA-binding surface affect RNA binding and signaling by RIG-I. These results provide the mechanistic basis for how RIG-I recognizes different RNA ligands.

PubMed: 20961956
DOI: 10.1093/nar/gkq974

Experimental method

X-RAY DIFFRACTION (2.4 Å)