3OE1

Pyruvate decarboxylase variant Glu473Asp from Z. mobilis in complex with reaction intermediate 2-lactyl-ThDP

Summary for 3OE1

Entry DOI	10.2210/pdb3oe1/pdb
Related	1ZPD
Descriptor	Pyruvate decarboxylase, MAGNESIUM ION, 3-[(4-AMINO-2-METHYLPYRIMIDIN-5-YL)METHYL]-2-(1-CARBOXY-1-HYDROXYETHYL)-5-(2-{[HYDROXY(PHOSPHONOOXY)PHOSPHORYL]OXY}ETHYL)-4-METHYL-1,3-THIAZOL-3-IUM, ... (5 entities in total)
Functional Keywords	lyase
Biological source	Zymomonas mobilis
Total number of polymer chains	4
Total formula weight	246240.10
Authors	Meyer, D.,Neumann, P.,Parthier, C.,Tittmann, K. (deposition date: 2010-08-12, release date: 2010-09-08, Last modification date: 2023-09-06)
Primary citation	Meyer, D.,Neumann, P.,Parthier, C.,Friedemann, R.,Nemeria, N.,Jordan, F.,Tittmann, K. Double duty for a conserved glutamate in pyruvate decarboxylase: evidence of the participation in stereoelectronically controlled decarboxylation and in protonation of the nascent carbanion/enamine intermediate . Biochemistry, 49:8197-8212, 2010 Cited by PubMed: 20715795 DOI: 10.1021/bi100828r PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.985 Å)

Structure validation