3NZI

Substrate induced remodeling of the active site regulates HtrA1 activity

3NZI の概要

• エントリーDOI: 10.2210/pdb3nzi/pdb
• 関連するPDBエントリー: 3NUM 3NWU
• 分子名称: Serine protease HTRA1, Citrate synthase (2 entities in total)
• 機能のキーワード: serine protease, degp, htra, protease, hydrolase-peptide inhibitor complex, hydrolase-hydrolase substrate complex, hydrolase/hydrolase substrate
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cell membrane : Q92743
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 37535.77

構造登録者

Truebestein, L.,Tennstaedt, A.,Hauske, P.,Krojer, T.,Kaiser, M.,Clausen, T.,Ehrmann, M. (登録日: 2010-07-16, 公開日: 2011-02-23, 最終更新日: 2024-11-20)

主引用文献

Truebestein, L.,Tennstaedt, A.,Monig, T.,Krojer, T.,Canellas, F.,Kaiser, M.,Clausen, T.,Ehrmann, M.
Substrate-induced remodeling of the active site regulates human HTRA1 activity.
Nat.Struct.Mol.Biol., 18:386-388, 2011

PubMed Abstract: Crystal structures of active and inactive conformations of the human serine protease HTRA1 reveal that substrate binding to the active site is sufficient to stimulate proteolytic activity. HTRA1 attaches to liposomes, digests misfolded proteins into defined fragments and undergoes substrate-mediated oligomer conversion. In contrast to those of other serine proteases, the PDZ domain of HTRA1 is dispensable for activation or lipid attachment, indicative of different underlying mechanistic features.

PubMed: 21297635
DOI: 10.1038/nsmb.2013

実験手法

X-RAY DIFFRACTION (2.75 Å)