3N7J

Crystal structure of botulinum neurotoxin serotype D binding domain

Summary for 3N7J

• Entry DOI: 10.2210/pdb3n7j/pdb
• Related: 3N7K 3N7L 3N7M
• Descriptor: Botulinum neurotoxin type D (2 entities in total)
• Functional Keywords: botulinum neurotoxin, hcr/d, ganglioside binding loop, toxin
• Biological source: Clostridium botulinum
• Cellular location: Botulinum neurotoxin D light chain: Secreted. Botulinum neurotoxin D heavy chain: Secreted: P19321
• Total number of polymer chains: 1
• Total formula weight: 48237.25

Authors

Fu, Z.,Baldwin, M.R.,Karalewitz, A.,Kroken, A.,Barbieri, J.T.,Kim, J.-J.P. (deposition date: 2010-05-27, release date: 2010-09-08, Last modification date: 2023-09-06)

Primary citation

Karalewitz, A.P.,Kroken, A.R.,Fu, Z.,Baldwin, M.R.,Kim, J.J.,Barbieri, J.T.
Identification of a Unique Ganglioside Binding Loop within Botulinum Neurotoxins C and D-SA .
Biochemistry, 49:8117-8126, 2010

PubMed Abstract: The botulinum neurotoxins (BoNTs) are the most potent protein toxins for humans. There are seven serotypes of BoNTs (A-G) based on a lack of cross antiserum neutralization. BoNTs utilize gangliosides as components of the host receptors for binding and entry into neurons. Members of BoNT/C and BoNT/D serotypes include mosaic toxins that are organized in D/C and C/D toxins. One D/C mosaic toxin, BoNT/D-South Africa (BoNT/D-SA), was not fully neutralized by immunization with BoNT serotype C or D, which stimulated this study. Here the crystal structures of the receptor binding domains of BoNT/C, BoNT/D, and BoNT/D-SA are presented. Biochemical and cell binding studies show that BoNT/C and BoNT/D-SA possess unique mechanisms for ganglioside binding. These studies provide new information about how the BoNTs can enter host cells as well as a basis for understanding the immunological diversity of these neurotoxins.

PubMed: 20731382
DOI: 10.1021/bi100865f

Experimental method

X-RAY DIFFRACTION (2 Å)