3MV7

Crystal Structure of the TK3 TCR in complex with HLA-B*3501/HPVG

3MV7 の概要

• エントリーDOI: 10.2210/pdb3mv7/pdb
• 関連するPDBエントリー: 2fyy 3MV8 3MV9
• 分子名称: HLA class I histocompatibility antigen, B-35 alpha chain, Beta-2-microglobulin, HPVG peptide from Epstein-Barr nuclear antigen 1, ... (7 entities in total)
• 機能のキーワード: hla b*3501, ebv, tcr, tcrpmhc complex, hpvg, tcr polymorphism, immune system
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Membrane; Single-pass type I membrane protein: P30685; Secreted: P61769; Host nucleus: P03211
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 94478.42

構造登録者

Gras, S.,Chen, Z.,Miles, J.J.,Liu, Y.C.,Bell, M.J.,Sullivan, L.C.,Kjer-Nielsen, L.,Brennan, R.M.,Burrows, J.M.,Neller, M.A.,Khanna, R.,Purcell, A.W.,Brooks, A.G.,McCluskey, J.,Rossjohn, J.,Burrows, S.R. (登録日: 2010-05-03, 公開日: 2010-06-09, 最終更新日: 2024-11-13)

主引用文献

Gras, S.,Chen, Z.,Miles, J.J.,Liu, Y.C.,Bell, M.J.,Sullivan, L.C.,Kjer-Nielsen, L.,Brennan, R.M.,Burrows, J.M.,Neller, M.A.,Khanna, R.,Purcell, A.W.,Brooks, A.G.,McCluskey, J.,Rossjohn, J.,Burrows, S.R.
Allelic polymorphism in the T cell receptor and its impact on immune responses
J.Exp.Med., 207:1555-1567, 2010

PubMed Abstract: In comparison to human leukocyte antigen (HLA) polymorphism, the impact of allelic sequence variation within T cell receptor (TCR) loci is much less understood. Particular TCR loci have been associated with autoimmunity, but the molecular basis for this phenomenon is undefined. We examined the T cell response to an HLA-B*3501-restricted epitope (HPVGEADYFEY) from Epstein-Barr virus (EBV), which is frequently dominated by a TRBV9*01(+) public TCR (TK3). However, the common allelic variant TRBV9*02, which differs by a single amino acid near the CDR2beta loop (Gln55-->His55), was never used in this response. The structure of the TK3 TCR, its allelic variant, and a nonnaturally occurring mutant (Gln55-->Ala55) in complex with HLA-B*3501(HPVGEADYFEY) revealed that the Gln55-->His55 polymorphism affected the charge complementarity at the TCR-peptide-MHC interface, resulting in reduced functional recognition of the cognate and naturally occurring variants of this EBV peptide. Thus, polymorphism in the TCR loci may contribute toward variability in immune responses and the outcome of infection.

PubMed: 20566715
DOI: 10.1084/jem.20100603

実験手法

X-RAY DIFFRACTION (2 Å)