3MU7

Crystal structure of the xylanase and alpha-amylase inhibitor protein (XAIP-II) from scadoxus multiflorus at 1.2 A resolution

Summary for 3MU7

• Entry DOI: 10.2210/pdb3mu7/pdb
• Related: 3HU7
• Descriptor: xylanase and alpha-amylase inhibitor protein, PHOSPHATE ION, DI(HYDROXYETHYL)ETHER, ... (4 entities in total)
• Functional Keywords: tim barell, amylase/xylanase inhibitory protein, hydrolase inhibitor
• Biological source: Scadoxus multiflorus
• Total number of polymer chains: 1
• Total formula weight: 30370.81

Authors

Kumar, S.,Singh, N.,Sinha, M.,Kaur, P.,Sharma, S.,Singh, T.P. (deposition date: 2010-05-02, release date: 2010-07-07, Last modification date: 2024-10-30)

Primary citation

Kumar, S.,Singh, N.,Mishra, B.,Dube, D.,Sinha, M.,Singh, S.B.,Dey, S.,Kaur, P.,Sharma, S.,Singh, T.P.
Modulation of inhibitory activity of xylanase-alpha-amylase inhibitor protein (XAIP): binding studies and crystal structure determination of XAIP-II from Scadoxus multiflorus at 1.2 A resolution.
Bmc Struct.Biol., 10:41-41, 2010

PubMed Abstract: Plants produce a wide range of proteinaceous inhibitors to protect themselves against hydrolytic enzymes. Recently a novel protein XAIP belonging to a new sub-family (GH18C) was reported to inhibit two structurally unrelated enzymes xylanase GH11 and α-amylase GH13. It was shown to inhibit xylanase GH11 with greater potency than that of α-amylase GH13. A new form of XAIP (XAIP-II) that inhibits α-amylase GH13 with a greater potency than that of XAIP and xylanase GH11 with a lower potency than that of XAIP, has been identified in the extracts of underground bulbs of Scadoxus multiflorus. This kind of occurrence of isoforms of inhibitor proteins is a rare observation and offers new opportunities for understanding the principles of protein engineering by nature.

PubMed: 21092126
DOI: 10.1186/1472-6807-10-41

Experimental method

X-RAY DIFFRACTION (1.29 Å)