3MU7
Crystal structure of the xylanase and alpha-amylase inhibitor protein (XAIP-II) from scadoxus multiflorus at 1.2 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2009-07-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.152, 64.398, 48.594 |
| Unit cell angles | 90.00, 102.10, 90.00 |
Refinement procedure
| Resolution | 47.670 - 1.290 |
| R-factor | 0.15334 |
| Rwork | 0.153 |
| R-free | 0.17086 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hu7 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.312 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.670 | 1.310 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Number of reflections | 61161 | |
| Completeness [%] | 100.0 | 97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | PEG 4000, Sodium Acetate, Ammonium Sulphate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
