3MR5

Human DNA polymerase eta - DNA ternary complex with a CPD 1bp upstream of the active site (TT3)

3MR5 の概要

• エントリーDOI: 10.2210/pdb3mr5/pdb
• 関連するPDBエントリー: 3MR2 3MR3 3MR4 3MR6
• 分子名称: DNA polymerase eta, DNA (5'-D(*TP*AP*AP*CP*(TTD)P*AP*TP*GP*AP*CP*GP*A)-3'), DNA (5'-D(*AP*CP*GP*TP*CP*AP*TP*AP*A)-3'), ... (7 entities in total)
• 機能のキーワード: pol eta, polymerase, thymine dimer, cpd, xpv, xeroderma pigmentosum variant, dna damage, transferase-dna complex, transferase/dna
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: Q9Y253
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 57390.19

構造登録者

Biertumpfel, C.,Zhao, Y.,Ramon-Maiques, S.,Gregory, M.T.,Lee, J.Y.,Yang, W. (登録日: 2010-04-28, 公開日: 2010-06-30, 最終更新日: 2024-04-03)

主引用文献

Biertumpfel, C.,Zhao, Y.,Kondo, Y.,Ramon-Maiques, S.,Gregory, M.,Lee, J.Y.,Masutani, C.,Lehmann, A.R.,Hanaoka, F.,Yang, W.
Structure and mechanism of human DNA polymerase eta.
Nature, 465:1044-1048, 2010

PubMed Abstract: The variant form of the human syndrome xeroderma pigmentosum (XPV) is caused by a deficiency in DNA polymerase eta (Poleta), a DNA polymerase that enables replication through ultraviolet-induced pyrimidine dimers. Here we report high-resolution crystal structures of human Poleta at four consecutive steps during DNA synthesis through cis-syn cyclobutane thymine dimers. Poleta acts like a 'molecular splint' to stabilize damaged DNA in a normal B-form conformation. An enlarged active site accommodates the thymine dimer with excellent stereochemistry for two-metal ion catalysis. Two residues conserved among Poleta orthologues form specific hydrogen bonds with the lesion and the incoming nucleotide to assist translesion synthesis. On the basis of the structures, eight Poleta missense mutations causing XPV can be rationalized as undermining the molecular splint or perturbing the active-site alignment. The structures also provide an insight into the role of Poleta in replicating through D loop and DNA fragile sites.

PubMed: 20577208
DOI: 10.1038/nature09196

実験手法

X-RAY DIFFRACTION (1.8 Å)