3MDI

Crystal Structure of the 25kDa Subunit of Human Cleavage factor Im in complex with RNA UGUAAA

3MDI の概要

• エントリーDOI: 10.2210/pdb3mdi/pdb
• 関連するPDBエントリー: 2Cl3 2j8q 3BAP 3BHO 3MDG
• 分子名称: Cleavage and polyadenylation specificity factor subunit 5, RNA (5'-R(*UP*GP*UP*AP*AP*A)-3'), GLYCEROL, ... (4 entities in total)
• 機能のキーワード: cpsf5, cf im, mrna processing, cleavage factor, nudix protein, protein-rna complex, rna binding protein, rna binding-rna complex, rna binding/rna
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: O43809
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 54602.87

構造登録者

Yang, Q.,Gilmartin, G.M.,Doublie, S. (登録日: 2010-03-30, 公開日: 2010-06-02, 最終更新日: 2023-09-06)

主引用文献

Yang, Q.,Gilmartin, G.M.,Doublie, S.
Structural basis of UGUA recognition by the Nudix protein CFI(m)25 and implications for a regulatory role in mRNA 3' processing.
Proc.Natl.Acad.Sci.USA, 107:10062-10067, 2010

PubMed Abstract: Human Cleavage Factor Im (CFI(m)) is an essential component of the pre-mRNA 3' processing complex that functions in the regulation of poly(A) site selection through the recognition of UGUA sequences upstream of the poly(A) site. Although the highly conserved 25 kDa subunit (CFI(m)25) of the CFI(m) complex possesses a characteristic alpha/beta/alpha Nudix fold, CFI(m)25 has no detectable hydrolase activity. Here we report the crystal structures of the human CFI(m)25 homodimer in complex with UGUAAA and UUGUAU RNA sequences. CFI(m)25 is the first Nudix protein to be reported to bind RNA in a sequence-specific manner. The UGUA sequence contributes to binding specificity through an intramolecular G:A Watson-Crick/sugar-edge base interaction, an unusual pairing previously found to be involved in the binding specificity of the SAM-III riboswitch. The structures, together with mutational data, suggest a novel mechanism for the simultaneous sequence-specific recognition of two UGUA elements within the pre-mRNA. Furthermore, the mutually exclusive binding of RNA and the signaling molecule Ap(4)A (diadenosine tetraphosphate) by CFI(m)25 suggests a potential role for small molecules in the regulation of mRNA 3' processing.

PubMed: 20479262
DOI: 10.1073/pnas.1000848107

実験手法

X-RAY DIFFRACTION (2.07 Å)