3M94
Complex crystal structure of Ascaris suum eIF4E-3 with m2,2,7G cap
Summary for 3M94
| Entry DOI | 10.2210/pdb3m94/pdb |
| Related | 3M93 |
| Descriptor | Translation initiation factor 4E, Eukaryotic translation initiation factor 4E-binding protein 1, N,N,7-trimethylguanosine 5'-(trihydrogen diphosphate), ... (5 entities in total) |
| Functional Keywords | eif4e, berkeley structural genomics center, bsgc, translation |
| Biological source | Ascaris suum (Pig roundworm) More |
| Total number of polymer chains | 2 |
| Total formula weight | 24845.24 |
| Authors | Liu, W.,Berkeley Structural Genomics Center (BSGC) (deposition date: 2010-03-19, release date: 2011-07-20, Last modification date: 2023-09-06) |
| Primary citation | Liu, W.,Jankowska-Anyszka, M.,Piecyk, K.,Dickson, L.,Wallace, A.,Niedzwiecka, A.,Stepinski, J.,Stolarski, R.,Darzynkiewicz, E.,Kieft, J.,Zhao, R.,Jones, D.N.,Davis, R.E. Structural basis for nematode eIF4E binding an m2,2,7G-Cap and its implications for translation initiation. Nucleic Acids Res., 39:8820-8832, 2011 Cited by PubMed Abstract: Metazoan spliced leader (SL) trans-splicing generates mRNAs with an m(2,2,7)G-cap and a common downstream SL RNA sequence. The mechanism for eIF4E binding an m²²⁷G-cap is unknown. Here, we describe the first structure of an eIF4E with an m(2,2,7)G-cap and compare it to the cognate m⁷G-eIF4E complex. These structures and Nuclear Magnetic Resonance (NMR) data indicate that the nematode Ascaris suum eIF4E binds the two different caps in a similar manner except for the loss of a single hydrogen bond on binding the m(2,2,7)G-cap. Nematode and mammalian eIF4E both have a low affinity for m(2,2,7)G-cap compared with the m⁷G-cap. Nematode eIF4E binding to the m⁷G-cap, m(2,2,7)G-cap and the m(2,2,7)G-SL 22-nt RNA leads to distinct eIF4E conformational changes. Additional interactions occur between Ascaris eIF4E and the SL on binding the m(2,2,7)G-SL. We propose interactions between Ascaris eIF4E and the SL impact eIF4G and contribute to translation initiation, whereas these interactions do not occur when only the m(2,2,7)G-cap is present. These data have implications for the contribution of 5'-UTRs in mRNA translation and the function of different eIF4E isoforms. PubMed: 21965542DOI: 10.1093/nar/gkr650 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.05 Å) |
Structure validation
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