3M3J
A new crystal form of Lys48-linked diubiquitin
Summary for 3M3J
| Entry DOI | 10.2210/pdb3m3j/pdb |
| Descriptor | Ubiquitin, 1,2-ETHANEDIOL, SULFATE ION, ... (4 entities in total) |
| Functional Keywords | beta grasp, ubiquitin, isopeptide bond, nucleus, protein degradation, proteasome, signaling protein, lys48-linked |
| Biological source | Bos taurus (bovine,cow,domestic cattle,domestic cow) |
| Total number of polymer chains | 6 |
| Total formula weight | 51811.24 |
| Authors | Trempe, J.F.,Brown, N.R.,Noble, M.E.M.,Endicott, J.A. (deposition date: 2010-03-09, release date: 2010-03-23, Last modification date: 2024-11-20) |
| Primary citation | Trempe, J.F.,Brown, N.R.,Noble, M.E.,Endicott, J.A. A new crystal form of Lys48-linked diubiquitin. Acta Crystallogr.,Sect.F, 66:994-998, 2010 Cited by PubMed Abstract: Lys48-linked polyubiquitin chains are recognized by the proteasome as a tag for the degradation of the attached substrates. Here, a new crystal form of Lys48-linked diubiquitin (Ub2) was obtained and the crystal structure was refined to 1.6 A resolution. The structure reveals an ordered isopeptide bond in a trans configuration. All three molecules in the asymmetric unit were in the same closed conformation, in which the hydrophobic patches of both the distal and the proximal moieties interact with each other. Despite the different crystallization conditions and different crystal packing, the new crystal structure of Ub2 is similar to the previously published structure of diubiquitin, but differences are observed in the conformation of the flexible isopeptide linkage. PubMed: 20823512DOI: 10.1107/S1744309110027600 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.6 Å) |
Structure validation
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