3L0F

High resolution structure of C-Phycocyanin from Thermosynechococcus elongatus

Summary for 3L0F

• Entry DOI: 10.2210/pdb3l0f/pdb
• Related: 1F99 1JB0 1KTP 1PHN 2BV8 3BRP
• Descriptor: C-phycocyanin alpha chain, C-phycocyanin beta chain, PHYCOCYANOBILIN, ... (4 entities in total)
• Functional Keywords: photosynthesis, photosystem ii, light harvesting proteins, thermostability, bile pigment, chloroplast, chromophore, electron transport, membrane, phycobilisome, plastid, thylakoid, transport, methylation
• Biological source: Thermosynechococcus elongatus; More
• Cellular location: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side (By similarity): P50032 P50033
• Total number of polymer chains: 2
• Total formula weight: 37439.36

Authors

Fromme, R.,Brune, D.,Fromme, P. (deposition date: 2009-12-09, release date: 2010-12-29, Last modification date: 2023-09-20)

Primary citation

Fromme, R.,Ishchenko, A.,Metz, M.,Chowdhury, S.R.,Basu, S.,Boutet, S.,Fromme, P.,White, T.A.,Barty, A.,Spence, J.C.,Weierstall, U.,Liu, W.,Cherezov, V.
Serial femtosecond crystallography of soluble proteins in lipidic cubic phase.
Iucrj, 2:545-551, 2015

PubMed Abstract: Serial femtosecond crystallography (SFX) at X-ray free-electron lasers (XFELs) enables high-resolution protein structure determination using micrometre-sized crystals at room temperature with minimal effects from radiation damage. SFX requires a steady supply of microcrystals intersecting the XFEL beam at random orientations. An LCP-SFX method has recently been introduced in which microcrystals of membrane proteins are grown and delivered for SFX data collection inside a gel-like membrane-mimetic matrix, known as lipidic cubic phase (LCP), using a special LCP microextrusion injector. Here, it is demonstrated that LCP can also be used as a suitable carrier medium for microcrystals of soluble proteins, enabling a dramatic reduction in the amount of crystallized protein required for data collection compared with crystals delivered by liquid injectors. High-quality LCP-SFX data sets were collected for two soluble proteins, lysozyme and phycocyanin, using less than 0.1 mg of each protein.

PubMed: 26306196
DOI: 10.1107/S2052252515013160

Experimental method

X-RAY DIFFRACTION (1.35 Å)