2BV8

The crystal structure of Phycocyanin from Gracilaria chilensis.

Summary for 2BV8

• Entry DOI: 10.2210/pdb2bv8/pdb
• Descriptor: C-PHYCOCYANIN ALPHA SUBUNIT, C-PHYCOCYANIN BETA SUBUNIT, PHYCOCYANOBILIN, ... (5 entities in total)
• Functional Keywords: light harvesting, phycobilisome, phycobiliprotein, phycocyanin, phycocyanobilin, photosynthesis, chloroplast
• Biological source: GRACILARIA CHILENSIS (RED ALGA); More
• Total number of polymer chains: 12
• Total formula weight: 225287.38

Authors

Contreras-Martel, C.,Martinez-Oyanedel, J.,Poo-Caama, G.,Bruna, C.,Bunster, M. (deposition date: 2005-06-23, release date: 2006-08-16, Last modification date: 2023-12-13)

Primary citation

Contreras-Martel, C.,Matamala, A.,Bruna, C.,Poo-Caama, G.,Almonacid, D.,Figueroa, M.,Martinez-Oyanedel, J.,Bunster, M.
The Structure at 2 A Resolution of Phycocyanin from Gracilaria Chilensis and the Energy Transfer Network in a Pc-Pc Complex.
Biophys.Chem., 125:388-, 2007

PubMed Abstract: Phycocyanin is a phycobiliprotein involved in light harvesting and conduction of light to the reaction centers in cyanobacteria and red algae. The structure of C-phycocyanin from Gracilaria chilensis was solved by X-ray crystallography at 2.0 A resolution in space group P2(1). An interaction model between two PC heterohexamers was built, followed by molecular dynamic refinement. The best model showed an inter-hexamer rotation of 23 degrees . The coordinates of a PC heterohexamer (alphabeta)(6) and of the PC-PC complex were used to perform energy transfer calculations between chromophores pairs using the fluorescence resonance energy transfer approach (FRET). Two main intra PC ((I)beta(3)(82)-->(I)alpha(1)(84)-->(I)alpha(5)(84)-->(I)beta(6)(82) and (I)beta(3)(153)-->(I)beta(5)(153)) and two main inter PC ((I)beta(6)(82)-->(II)beta(3)(82) and (I)beta(5)(153)-->(II)beta(3)(153)) pathways were proposed based on the values of the energy transfer constants calculated for all the chromophore pairs in the hexamer and in the complex.

PubMed: 17118524
DOI: 10.1016/J.BPC.2006.09.014

Experimental method

X-RAY DIFFRACTION (2.01 Å)