3KH2

Crystal structure of the P1 bacteriophage Doc toxin (F68S) in complex with the Phd antitoxin (L17M/V39A). Northeast Structural Genomics targets ER385-ER386

Summary for 3KH2

• Entry DOI: 10.2210/pdb3kh2/pdb
• Descriptor: Death on curing protein, Prevent host death protein, CHLORIDE ION, ... (7 entities in total)
• Functional Keywords: toxin, antitoxin, structural genomics, psi-2, northeast structural genomics consortium (nesg), protein structure initiative
• Biological source: Bacteriophage P1; More
• Total number of polymer chains: 8
• Total formula weight: 92739.91

Authors

Arbing, M.A.,Kuzin, A.P.,Su, M.,Abashidze, M.,Verdon, G.,Liu, M.,Xiao, R.,Acton, T.,Inouye, M.,Montelione, G.T.,Woychik, N.A.,Hunt, J.F.,Northeast Structural Genomics Consortium (NESG) (deposition date: 2009-10-29, release date: 2010-08-18, Last modification date: 2024-10-30)

Primary citation

Arbing, M.A.,Handelman, S.K.,Kuzin, A.P.,Verdon, G.,Wang, C.,Su, M.,Rothenbacher, F.P.,Abashidze, M.,Liu, M.,Hurley, J.M.,Xiao, R.,Acton, T.,Inouye, M.,Montelione, G.T.,Woychik, N.A.,Hunt, J.F.
Crystal Structures of Phd-Doc, HigA, and YeeU Establish Multiple Evolutionary Links between Microbial Growth-Regulating Toxin-Antitoxin Systems.
Structure, 18:996-1010, 2010

PubMed Abstract: Bacterial toxin-antitoxin (TA) systems serve a variety of physiological functions including regulation of cell growth and maintenance of foreign genetic elements. Sequence analyses suggest that TA families are linked by complex evolutionary relationships reflecting likely swapping of functional domains between different TA families. Our crystal structures of Phd-Doc from bacteriophage P1, the HigA antitoxin from Escherichia coli CFT073, and YeeU of the YeeUWV systems from E. coli K12 and Shigella flexneri confirm this inference and reveal additional, unanticipated structural relationships. The growth-regulating Doc toxin exhibits structural similarity to secreted virulence factors that are toxic for eukaryotic target cells. The Phd antitoxin possesses the same fold as both the YefM and NE2111 antitoxins that inhibit structurally unrelated toxins. YeeU, which has an antitoxin-like activity that represses toxin expression, is structurally similar to the ribosome-interacting toxins YoeB and RelE. These observations suggest extensive functional exchanges have occurred between TA systems during bacterial evolution.

PubMed: 20696400
DOI: 10.1016/j.str.2010.04.018

Experimental method

X-RAY DIFFRACTION (2.71 Å)