3KH2
Crystal structure of the P1 bacteriophage Doc toxin (F68S) in complex with the Phd antitoxin (L17M/V39A). Northeast Structural Genomics targets ER385-ER386
Entity
Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
1 | A, B, C, D | Death on curing protein | polymer | 134 | 14708.1 | 4 | UniProt (Q06259) Pfam (PF02661) In PDB | Bacteriophage P1 | |
2 | E, F, G, H | Prevent host death protein | polymer | 73 | 8226.9 | 4 | UniProt (Q06253) Pfam (PF02604) In PDB | Bacteriophage P1 | |
3 | A | CHLORIDE ION | non-polymer | 35.5 | 1 | Chemie (CL) | |||
4 | A, B | 2-HYDROXYETHYL DISULFIDE | non-polymer | 154.3 | 2 | Chemie (HED) | |||
5 | B, H | GLYCEROL | non-polymer | 92.1 | 3 | Chemie (GOL) | |||
6 | E, F, G, H | PHOSPHATE ION | non-polymer | 95.0 | 4 | Chemie (PO4) | |||
7 | water | water | 18.0 | 93 | Chemie (HOH) |
Sequence modifications
A, B, C, D: 2 - 126 (UniProt: Q06259)
E, F, G, H: 1 - 73 (UniProt: Q06253)
PDB | External Database | Details |
---|---|---|
Mse 1 | - | initiating methionine |
Ser 68 | Phe 68 | engineered mutation |
Leu 127 | - | expression tag |
Glu 128 | - | expression tag |
His 129 | - | expression tag |
His 130 | - | expression tag |
His 131 | - | expression tag |
His 132 | - | expression tag |
His 133 | - | expression tag |
His 134 | - | expression tag |
PDB | External Database | Details |
---|---|---|
Mse 17 | Leu 17 | engineered mutation |
Ala 39 | Val 39 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
Polymers | Number of chains | 8 |
Total formula weight | 91739.8 | |
Non-Polymers* | Number of molecules | 10 |
Total formula weight | 1000.1 | |
All* | Total formula weight | 92739.9 |