3K74
Disruption of protein dynamics by an allosteric effector antibody
Summary for 3K74
| Entry DOI | 10.2210/pdb3k74/pdb |
| Related | 1HCV 5DFR |
| Descriptor | Dihydrofolate reductase, Nanobody (3 entities in total) |
| Functional Keywords | immunoglobulin, protein-nanobody complex, antibiotic resistance, methotrexate resistance, nadp, one-carbon metabolism, oxidoreductase, trimethoprim resistance |
| Biological source | Escherichia coli K-12 More |
| Total number of polymer chains | 2 |
| Total formula weight | 30570.49 |
| Authors | Oyen, D.,Srinivasan, V.,Steyaert, J.,Barlow, J. (deposition date: 2009-10-12, release date: 2010-10-20, Last modification date: 2024-11-06) |
| Primary citation | Oyen, D.,Srinivasan, V.,Steyaert, J.,Barlow, J.N. Constraining enzyme conformational change by an antibody leads to hyperbolic inhibition. J.Mol.Biol., 407:138-148, 2011 Cited by PubMed Abstract: Although it has been known for many years that antibodies display properties characteristic of allosteric effectors, the molecular mechanisms responsible for these effects remain poorly understood. Here, we describe a single-domain antibody fragment (nanobody) that modulates protein function by constraining conformational change in the enzyme dihydrofolate reductase (DHFR). Nanobody 216 (Nb216) behaves as a potent allosteric inhibitor of DHFR, giving rise to mixed hyperbolic inhibition kinetics. The crystal structure of Nb216 in complex with DHFR reveals that the nanobody binds adjacent to the active site. Half of the epitope consists of residues from the flexible Met20 loop. This loop, which ordinarily oscillates between occluded and closed conformations during catalysis, assumes the occluded conformation in the Nb216-bound state. Using stopped flow, we show that Nb216 inhibits DHFR by stabilising the occluded Met20 loop conformation. Surprisingly, kinetic data indicate that the Met20 loop retains sufficient conformational flexibility in the Nb216-bound state to allow slow substrate turnover to occur. PubMed: 21238460DOI: 10.1016/j.jmb.2011.01.017 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.95 Å) |
Structure validation
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