3K4X

Eukaryotic Sliding Clamp PCNA Bound to DNA

Summary for 3K4X

• Entry DOI: 10.2210/pdb3k4x/pdb
• Descriptor: Proliferating cell nuclear antigen, DNA (5'-D(*CP*CP*CP*AP*TP*CP*GP*TP*AP*T)-3'), DNA (5'-D(*TP*TP*TP*TP*AP*TP*AP*CP*GP*AP*TP*GP*GP*G)-3') (3 entities in total)
• Functional Keywords: dna replication, dna-binding, clamp loader, dna sliding clamp, dna binding protein-dna complex, dna binding protein/dna
• Biological source: Saccharomyces cerevisiae (brewer's yeast,lager beer yeast,yeast); More
• Cellular location: Nucleus: P15873
• Total number of polymer chains: 3
• Total formula weight: 96070.52

Authors

McNally, R.,Kuriyan, J. (deposition date: 2009-10-06, release date: 2010-02-16, Last modification date: 2023-09-06)

Primary citation

McNally, R.,Bowman, G.D.,Goedken, E.R.,O'Donnell, M.,Kuriyan, J.
Analysis of the role of PCNA-DNA contacts during clamp loading.
Bmc Struct.Biol., 10:3-3, 2010

PubMed Abstract: Sliding clamps, such as Proliferating Cell Nuclear Antigen (PCNA) in eukaryotes, are ring-shaped protein complexes that encircle DNA and enable highly processive DNA replication by serving as docking sites for DNA polymerases. In an ATP-dependent reaction, clamp loader complexes, such as the Replication Factor-C (RFC) complex in eukaryotes, open the clamp and load it around primer-template DNA.

PubMed: 20113510
DOI: 10.1186/1472-6807-10-3

Experimental method

X-RAY DIFFRACTION (2.98 Å)