3K4X

Eukaryotic Sliding Clamp PCNA Bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	77
Detector technology	CCD
Collection date	2008-10-14
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	83.935, 93.712, 136.883
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.856 - 2.980
R-factor	0.226
Rwork	0.223
R-free	0.28000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1plq
RMSD bond length	0.003
RMSD bond angle	0.667
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.4_162)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	2.980	2.980
Rmerge	0.148	0.574
Number of reflections	22002
<I/σ(I)>	10.7	1.9
Completeness [%]	97.9	88.2
Redundancy	5.2	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	100 mM Na Acetate, 100 mM NaCl, 14% PEG 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K