3K3A

Crystal Structure of B/Perth Neuraminidase D197E mutant in complex with Oseltamivir

Summary for 3K3A

• Entry DOI: 10.2210/pdb3k3a/pdb
• Related: 3K36 3K37 3K38 3K39
• Descriptor: Neuraminidase, 2-acetamido-2-deoxy-beta-D-glucopyranose, CALCIUM ION, ... (6 entities in total)
• Functional Keywords: influenza, neuraminidase, mutation, resistance, tamiflu, oseltamivir, gs-4071, 196618-13-0, hydrolase, cell membrane, glycosidase, membrane, transmembrane, virion
• Biological source: Influenza B virus (Viruses)
• Total number of polymer chains: 16
• Total formula weight: 710507.62

Authors

Oakley, A.J.,McKimm-Breschkin, J.L. (deposition date: 2009-10-02, release date: 2010-09-01, Last modification date: 2024-10-30)

Primary citation

Oakley, A.J.,Barrett, S.,Peat, T.S.,Newman, J.,Streltsov, V.A.,Waddington, L.,Saito, T.,Tashiro, M.,McKimm-Breschkin, J.L.
Structural and Functional Basis of Resistance to Neuraminidase Inhibitors of Influenza B Viruses.
J.Med.Chem., 2010

PubMed Abstract: We have identified a virus, B/Perth/211/2001, with a spontaneous mutation, D197E in the neuraminidase (NA), which confers cross-resistance to all NA inhibitors. We analyzed enzyme properties of the D197 and E197 NAs and compared these to a D197N NA, known to arise after oseltamivir treatment. Zanamivir and peramivir bound slowly to the wild type NA, but binding of oseltamivir was more rapid. The D197E/N mutations resulted in faster binding of all three inhibitors. Analysis of the crystal structures of D197 and E197 NAs with and without inhibitors showed that the D197E mutation compromised the interaction of neighboring R150 with the N-acetyl group, common to the substrate sialic acid and all NA inhibitors. Although rotation of the E275 in the NA active site occurs upon binding peramivir in both the D197 and E197 NAs, this does not occur upon binding oseltamivir in the E197 NA. Lack of the E275 rotation would also account for the loss of slow binding and the partial resistance of influenza B wild type NAs to oseltamivir.

PubMed: 20695427
DOI: 10.1021/jm100621s

Experimental method

X-RAY DIFFRACTION (2.59 Å)