3JTH
Crystal structure of a transcriptional regulator HlyU from Vibrio vulnificus CMCP6
Summary for 3JTH
| Entry DOI | 10.2210/pdb3jth/pdb |
| Descriptor | Transcription activator HlyU (2 entities in total) |
| Functional Keywords | transcription factor, hlyu, rtxa, dna-binding, transcription, transcription regulation |
| Biological source | Vibrio vulnificus |
| Total number of polymer chains | 2 |
| Total formula weight | 22903.60 |
| Authors | Nishi, K.,Lee, H.J.,Park, S.Y.,Kim, J.S. (deposition date: 2009-09-12, release date: 2010-03-16, Last modification date: 2024-11-13) |
| Primary citation | Nishi, K.,Lee, H.J.,Park, S.Y.,Bae, S.J.,Lee, S.E.,Adams, P.D.,Rhee, J.H.,Kim, J.S. Crystal structure of the transcriptional activator HlyU from Vibrio vulnificus CMCP6. Febs Lett., 2010 Cited by PubMed Abstract: HlyU is a transcription factor of the ArsR/SmtB family and activates the expression of the pathogenic Vibrio vulnificus RTX toxin. In contrast to the other metal-responding ArsR/SmtB proteins, HlyU does not sense metal ions. To provide its structural information, we elucidated the crystal structure of HlyU from V. vulnificus CMCP6 (HlyU_Vv). The monomeric HlyU_Vv architecture of five alpha-helices and two beta-strands, some of which constitute a typical DNA-binding winged helix-turn-helix (wHTH) motif, is very similar to that of other transcription regulators. Nonetheless, the homo-dimeric HlyU_Vv structure shows several different, three-dimensional features in the spatial position and the detailed dimeric interaction, which were not observed in the modeling study based on the same protein family and sequence similarity. PubMed: 20178784DOI: 10.1016/j.febslet.2010.02.052 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2 Å) |
Structure validation
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