3JTH
Crystal structure of a transcriptional regulator HlyU from Vibrio vulnificus CMCP6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 6C1 |
| Synchrotron site | PAL/PLS |
| Beamline | 6C1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-01-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.946, 52.790, 47.862 |
| Unit cell angles | 90.00, 103.92, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.000 |
| R-factor | 0.242 |
| Rwork | 0.231 |
| R-free | 0.29000 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.110 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.078 | 0.269 |
| Number of reflections | 12896 | |
| <I/σ(I)> | 2.94 | |
| Completeness [%] | 94.0 | 76.3 |
| Redundancy | 4.1 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 9~11% polyethylene glycol 20000, 0.1M Sodium Acetate, 0.1M (N-Morpholino) propanesulfonic acid, pH6.5, 10mM b-mercaptoethanol, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
