Summary for 3JBC
| Entry DOI | 10.2210/pdb3jbc/pdb |
| Related | 3JBD 3JBE 3JBF 3JBG |
| EMDB information | 5886 5888 6433 6434 6435 |
| Descriptor | Capsid protein VP1, Capsid protein VP2, Capsid protein VP3, ... (6 entities in total) |
| Functional Keywords | vhh, nanobody, poliovirus, virus-immune system complex, virus/immune system |
| Biological source | Camelus dromedarius (Arabian camel) More |
| Total number of polymer chains | 5 |
| Total formula weight | 112232.45 |
| Authors | Strauss, M.,Schotte, L.,Thys, B.,Filman, D.J.,Hogle, J.M. (deposition date: 2015-08-26, release date: 2016-01-27, Last modification date: 2022-12-21) |
| Primary citation | Strauss, M.,Schotte, L.,Thys, B.,Filman, D.J.,Hogle, J.M. Five of Five VHHs Neutralizing Poliovirus Bind the Receptor-Binding Site. J.Virol., 90:3496-3505, 2016 Cited by PubMed Abstract: Nanobodies, or VHHs, that recognize poliovirus type 1 have previously been selected and characterized as candidates for antiviral agents or reagents for standardization of vaccine quality control. In this study, we present high-resolution cryo-electron microscopy reconstructions of poliovirus with five neutralizing VHHs. All VHHs bind the capsid in the canyon at sites that extensively overlap the poliovirus receptor-binding site. In contrast, the interaction involves a unique (and surprisingly extensive) surface for each of the five VHHs. Five regions of the capsid were found to participate in binding with all five VHHs. Four of these five regions are known to alter during the expansion of the capsid associated with viral entry. Interestingly, binding of one of the VHHs, PVSS21E, resulted in significant changes of the capsid structure and thus seems to trap the virus in an early stage of expansion. PubMed: 26764003DOI: 10.1128/JVI.03017-15 PDB entries with the same primary citation |
| Experimental method | ELECTRON MICROSCOPY (5.6 Å) |
Structure validation
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