3JB9

Cryo-EM structure of the yeast spliceosome at 3.6 angstrom resolution

3JB9 の概要

• エントリーDOI: 10.2210/pdb3jb9/pdb
• EMDBエントリー: 6413
• 分子名称: Pre-mRNA-splicing factor spp42, Small nuclear ribonucleoprotein G, Pre-mRNA-splicing factor prp5, ... (37 entities in total)
• 機能のキーワード: spliceosome, u2/u5/u6, lariat, rna binding protein-rna complex, rna binding protein/rna
• 由来する生物種: Schizosaccharomyces pombe 972h- (Fission yeast); 詳細
• タンパク質・核酸の鎖数: 43
• 化学式量合計: 1789825.08

構造登録者

Yan, C.,Hang, J.,Wan, R.,Huang, M.,Wong, C.,Shi, Y. (登録日: 2015-08-09, 公開日: 2015-09-23, 最終更新日: 2024-03-20)

主引用文献

Yan, C.,Hang, J.,Wan, R.,Huang, M.,Wong, C.,Shi, Y.
Structure of a yeast spliceosome at 3.6-angstrom resolution
Science, 349:1182-1191, 2015

PubMed Abstract: Splicing of precursor messenger RNA (pre-mRNA) in yeast is executed by the spliceosome, which consists of five small nuclear ribonucleoproteins (snRNPs), NTC (nineteen complex), NTC-related proteins (NTR), and a number of associated enzymes and cofactors. Here, we report the three-dimensional structure of a Schizosaccharomyces pombe spliceosome at 3.6-angstrom resolution, revealed by means of single-particle cryogenic electron microscopy. This spliceosome contains U2 and U5 snRNPs, NTC, NTR, U6 small nuclear RNA, and an RNA intron lariat. The atomic model includes 10,574 amino acids from 37 proteins and four RNA molecules, with a combined molecular mass of approximately 1.3 megadaltons. Spp42 (Prp8 in Saccharomyces cerevisiae), the key protein component of the U5 snRNP, forms a central scaffold and anchors the catalytic center. Both the morphology and the placement of protein components appear to have evolved to facilitate the dynamic process of pre-mRNA splicing. Our near-atomic-resolution structure of a central spliceosome provides a molecular framework for mechanistic understanding of pre-mRNA splicing.

PubMed: 26292707
DOI: 10.1126/science.aac7629

実験手法

ELECTRON MICROSCOPY (3.6 Å)