3J9W

Cryo-EM structure of the Bacillus subtilis MifM-stalled ribosome complex

This is a non-PDB format compatible entry.

Summary for 3J9W

• Entry DOI: 10.2210/pdb3j9w/pdb
• EMDB information: 6306
• Descriptor: 16S ribosomal RNA, 30S ribosomal protein uS10, 30S ribosomal protein uS11, ... (54 entities in total)
• Functional Keywords: stalling, translation arrest, mifm, l22, ribosome
• Biological source: Bacillus subtilis subsp. subtilis str. 168; More
• Total number of polymer chains: 54
• Total formula weight: 2185398.95

Authors

Sohmen, D.,Chiba, S.,Shimokawa-Chiba, N.,Innis, C.A.,Berninghausen, O.,Beckmann, R.,Ito, K.,Wilson, D.N. (deposition date: 2015-03-16, release date: 2015-04-29, Last modification date: 2024-02-21)

Primary citation

Sohmen, D.,Chiba, S.,Shimokawa-Chiba, N.,Innis, C.A.,Berninghausen, O.,Beckmann, R.,Ito, K.,Wilson, D.N.
Structure of the Bacillus subtilis 70S ribosome reveals the basis for species-specific stalling.
Nat Commun, 6:6941-6941, 2015

PubMed Abstract: Ribosomal stalling is used to regulate gene expression and can occur in a species-specific manner. Stalling during translation of the MifM leader peptide regulates expression of the downstream membrane protein biogenesis factor YidC2 (YqjG) in Bacillus subtilis, but not in Escherichia coli. In the absence of structures of Gram-positive bacterial ribosomes, a molecular basis for species-specific stalling has remained unclear. Here we present the structure of a Gram-positive B. subtilis MifM-stalled 70S ribosome at 3.5-3.9 Å, revealing a network of interactions between MifM and the ribosomal tunnel, which stabilize a non-productive conformation of the PTC that prevents aminoacyl-tRNA accommodation and thereby induces translational arrest. Complementary genetic analyses identify a single amino acid within ribosomal protein L22 that dictates the species specificity of the stalling event. Such insights expand our understanding of how the synergism between the ribosome and the nascent chain is utilized to modulate the translatome in a species-specific manner.

PubMed: 25903689
DOI: 10.1038/ncomms7941

Experimental method

ELECTRON MICROSCOPY (3.9 Å)