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3J80

CryoEM structure of 40S-eIF1-eIF1A preinitiation complex

Summary for 3J80
Entry DOI10.2210/pdb3j80/pdb
Related3J81
EMDB information2764
Descriptor18S rRNA, uS17, uS15, ... (39 entities in total)
Functional Keywordssmall ribosome subunit, eukaryotic translation initiation, ribosome
Biological sourceSaccharomyces cerevisiae (yeast)
More
Total number of polymer chains37
Total formula weight1208132.06
Authors
Hussain, T.,Llacer, J.L.,Fernandez, I.S.,Savva, C.G.,Ramakrishnan, V. (deposition date: 2014-08-28, release date: 2014-11-05, Last modification date: 2024-02-21)
Primary citationHussain, T.,Llacer, J.L.,Fernandez, I.S.,Munoz, A.,Martin-Marcos, P.,Savva, C.G.,Lorsch, J.R.,Hinnebusch, A.G.,Ramakrishnan, V.
Structural changes enable start codon recognition by the eukaryotic translation initiation complex.
Cell(Cambridge,Mass.), 159:597-607, 2014
Cited by
PubMed Abstract: During eukaryotic translation initiation, initiator tRNA does not insert fully into the P decoding site on the 40S ribosomal subunit. This conformation (POUT) is compatible with scanning mRNA for the AUG start codon. Base pairing with AUG is thought to promote isomerization to a more stable conformation (PIN) that arrests scanning and promotes dissociation of eIF1 from the 40S subunit. Here, we present a cryoEM reconstruction of a yeast preinitiation complex at 4.0 Å resolution with initiator tRNA in the PIN state, prior to eIF1 release. The structure reveals stabilization of the codon-anticodon duplex by the N-terminal tail of eIF1A, changes in the structure of eIF1 likely instrumental in its subsequent release, and changes in the conformation of eIF2. The mRNA traverses the entire mRNA cleft and makes connections to the regulatory domain of eIF2?, eIF1A, and ribosomal elements that allow recognition of context nucleotides surrounding the AUG codon.
PubMed: 25417110
DOI: 10.1016/j.cell.2014.10.001
PDB entries with the same primary citation
Experimental method
ELECTRON MICROSCOPY (3.75 Å)
Structure validation

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