3J6X

S. cerevisiae 80S ribosome bound with Taura syndrome virus (TSV) IRES, 5 degree rotation (Class II)

これはPDB形式変換不可エントリーです。

3J6X の概要

• エントリーDOI: 10.2210/pdb3j6x/pdb
• 関連するPDBエントリー: 3J6Y 3U5D
• EMDBエントリー: 5942 5943
• 分子名称: 25S ribosomal RNA, 60S ribosomal protein L7, 60S ribosomal protein L8, ... (80 entities in total)
• 機能のキーワード: translation initiation, ribosome, intergenic ires, tsv ires
• 由来する生物種: Saccharomyces cerevisiae (yeast); 詳細
• タンパク質・核酸の鎖数: 80
• 化学式量合計: 3194034.32

構造登録者

Koh, C.S.,Brilot, A.F.,Grigorieff, N.,Korostelev, A.A. (登録日: 2014-04-16, 公開日: 2014-06-11, 最終更新日: 2024-02-21)

主引用文献

Koh, C.S.,Brilot, A.F.,Grigorieff, N.,Korostelev, A.A.
Taura syndrome virus IRES initiates translation by binding its tRNA-mRNA-like structural element in the ribosomal decoding center.
Proc.Natl.Acad.Sci.USA, 111:9139-9144, 2014

PubMed Abstract: In cap-dependent translation initiation, the open reading frame (ORF) of mRNA is established by the placement of the AUG start codon and initiator tRNA in the ribosomal peptidyl (P) site. Internal ribosome entry sites (IRESs) promote translation of mRNAs in a cap-independent manner. We report two structures of the ribosome-bound Taura syndrome virus (TSV) IRES belonging to the family of Dicistroviridae intergenic IRESs. Intersubunit rotational states differ in these structures, suggesting that ribosome dynamics play a role in IRES translocation. Pseudoknot I of the IRES occupies the ribosomal decoding center at the aminoacyl (A) site in a manner resembling that of the tRNA anticodon-mRNA codon. The structures reveal that the TSV IRES initiates translation by a previously unseen mechanism, which is conceptually distinct from initiator tRNA-dependent mechanisms. Specifically, the ORF of the IRES-driven mRNA is established by the placement of the preceding tRNA-mRNA-like structure in the A site, whereas the 40S P site remains unoccupied during this initial step.

PubMed: 24927574
DOI: 10.1073/pnas.1406335111

実験手法

ELECTRON MICROSCOPY (6.1 Å)