4V88

The structure of the eukaryotic ribosome at 3.0 A resolution.

This is a non-PDB format compatible entry.

Summary for 4V88

• Entry DOI: 10.2210/pdb4v88/pdb
• Descriptor: 18S RIBOSOMAL RNA, 40S ribosomal protein S8-A, 40S ribosomal protein S9-A, ... (89 entities in total)
• Functional Keywords: translation, ribosome, 40s, 60s, 80s, ribosomal, ribosomal rna, rrna, ribosomal protein, stm1, eukaryotic ribosome, rna-protein complex
• Biological source: Saccharomyces cerevisiae; More
• Cellular location: Cytoplasm : P0CX39 O13516 Q08745 P0CX47 P48589 P05756 P06367 Q01855 P0CX51 P02407 P32905 P0CX55 P07280 P38701 P0C0V8 P0C0W1 P0CX29 P0CX31 Q3E792 P39938 P35997 P33442 Q3E7X9 P41057 P0CX33 P38011 P39015 P0CX45 P25443 P14126 P10664 P26321 Q02326 P05737 P17076 P05738 P41805 P0C0W9 Q12690 P05750 P36105 P05748 P26784 P05740 P0CX49 P0CX82 P0CX23 Q02753 P05749 P0CX41 P0CX35 P04449 P04456 P05743 P0C2H6 P02406 P05747 P14120 P0C2H8 P38061 P05744 P26783 P87262 P0CX84 P05745 P49166 P49167 P04650 P0CX86 P0CX27 P0CX37 P05317 P0CX25 P26786; Ubiquitin: Cytoplasm . 40S ribosomal protein S31: Cytoplasm : P05759; Ubiquitin: Cytoplasm . 60S ribosomal protein L40-A: Cytoplasm : P0CH08
• Total number of polymer chains: 162
• Total formula weight: 6757336.09

Authors

Ben-Shem, A.,Garreau de Loubresse, N.,Melnikov, S.,Jenner, L.,Yusupova, G.,Yusupov, M. (deposition date: 2011-10-11, release date: 2014-07-09, Last modification date: 2024-11-06)

Primary citation

Ben-Shem, A.,Garreau de Loubresse, N.,Melnikov, S.,Jenner, L.,Yusupova, G.,Yusupov, M.
The structure of the eukaryotic ribosome at 3.0 angstrom resolution.
Science, 334:1524-1529, 2011

PubMed Abstract: Ribosomes translate genetic information encoded by messenger RNA into proteins. Many aspects of translation and its regulation are specific to eukaryotes, whose ribosomes are much larger and intricate than their bacterial counterparts. We report the crystal structure of the 80S ribosome from the yeast Saccharomyces cerevisiae--including nearly all ribosomal RNA bases and protein side chains as well as an additional protein, Stm1--at a resolution of 3.0 angstroms. This atomic model reveals the architecture of eukaryote-specific elements and their interaction with the universally conserved core, and describes all eukaryote-specific bridges between the two ribosomal subunits. It forms the structural framework for the design and analysis of experiments that explore the eukaryotic translation apparatus and the evolutionary forces that shaped it.

PubMed: 22096102

Experimental method

X-RAY DIFFRACTION (3 Å)