4V88
The structure of the eukaryotic ribosome at 3.0 A resolution.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2010-07-21 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 436.430, 288.220, 305.080 |
| Unit cell angles | 90.00, 98.99, 90.00 |
Refinement procedure
| Resolution | 300.000 - 3.000 |
| R-factor | 0.1822 |
| Rwork | 0.181 |
| R-free | 0.22850 |
| Structure solution method | SAD |
| Starting model (for MR) | PDB ENTRIES 3O2Z 3O30 3O58 3O5H |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.672 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 300.000 | 3.100 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 1639309 | |
| <I/σ(I)> | 14.8 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 38.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | PEG 20K, KSCN, Mg Acetate, Tris-Acetate, Glycerol, Spermidine, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
