3J3X

Independent reconstruction of Mm-cpn cryo-EM density map from half dataset in the closed state (training map)

3J3X の概要

• エントリーDOI: 10.2210/pdb3j3x/pdb
• EMDBエントリー: 5645 5646
• 分子名称: Chaperonin (1 entity in total)
• 機能のキーワード: modeling, independent reconstruction, cryo-em model validation, chaperone
• 由来する生物種: Methanococcus maripaludis
• タンパク質・核酸の鎖数: 16
• 化学式量合計: 915389.25

構造登録者

DiMaio, F.,Zhang, J.,Chiu, W.,Baker, D. (登録日: 2013-05-02, 公開日: 2013-05-29, 最終更新日: 2024-02-21)

主引用文献

Dimaio, F.,Zhang, J.,Chiu, W.,Baker, D.
Cryo-EM model validation using independent map reconstructions.
Protein Sci., 22:865-868, 2013

PubMed Abstract: An increasing number of cryo-electron microscopy (cryo-EM) density maps are being generated with suitable resolution to trace the protein backbone and guide sidechain placement. Generating and evaluating atomic models based on such maps would be greatly facilitated by independent validation metrics for assessing the fit of the models to the data. We describe such a metric based on the fit of atomic models with independent test maps from single particle reconstructions not used in model refinement. The metric provides a means to determine the proper balance between the fit to the density and model energy and stereochemistry during refinement, and is likely to be useful in determining values of model building and refinement metaparameters quite generally.

PubMed: 23592445
DOI: 10.1002/pro.2267

実験手法

ELECTRON MICROSCOPY (4.3 Å)