3J1N

Cryo-EM map of a yeast minimal preinitiation complex interacting with the Mediator Head module

Summary for 3J1N

• Entry DOI: 10.2210/pdb3j1n/pdb
• Related: 3J1O
• EMDB information: 5407
• Descriptor: DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase II subunit RPABC5, DNA-directed RNA polymerase II subunit RPB11, ... (12 entities in total)
• Functional Keywords: transcription, mediator head module, preinitiation complex, transferase
• Biological source: Saccharomyces cerevisiae (yeast); More
• Total number of polymer chains: 12
• Total formula weight: 471725.23

Authors

Asturias, F.J.,Imasaki, T. (deposition date: 2012-03-29, release date: 2013-03-06, Last modification date: 2024-02-21)

Primary citation

Cai, G.,Chaban, Y.L.,Imasaki, T.,Kovacs, J.A.,Calero, G.,Penczek, P.A.,Takagi, Y.,Asturias, F.J.
Interaction of the mediator head module with RNA polymerase II.
Structure, 20:899-910, 2012

PubMed Abstract: Mediator, a large (21 polypeptides, MW ∼1 MDa) complex conserved throughout eukaryotes, plays an essential role in control of gene expression by conveying regulatory signals that influence the activity of the preinitiation complex. However, the precise mode of interaction between Mediator and RNA polymerase II (RNAPII), and the mechanism of regulation by Mediator remain elusive. We used cryo-electron microscopy and reconstituted in vitro transcription assays to characterize a transcriptionally-active complex including the Mediator Head module and components of a minimum preinitiation complex (RNAPII, TFIIF, TFIIB, TBP, and promoter DNA). Our results reveal how the Head interacts with RNAPII, affecting its conformation and function.

PubMed: 22579255
DOI: 10.1016/j.str.2012.02.023

Experimental method

ELECTRON MICROSCOPY (16 Å)