3J1N
Cryo-EM map of a yeast minimal preinitiation complex interacting with the Mediator Head module
EM specimen
Vitrification | Yes |
Instrument | HOMEMADE PLUNGER |
Method | Blot for ~2 sec before plunging |
EM Data Collection
Microscope | FEI TECNAI F20 |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 120 |
Cs (mm) | 2 |
Detector | TVIPS TEMCAM-F415 (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 16 |
Software | OTHER,SPARX,SPIDER |
Symmetry | C1 |