3IPV

Crystal structure of Spatholobus parviflorus seed lectin

Summary for 3IPV

• Entry DOI: 10.2210/pdb3ipv/pdb
• Descriptor: Lectin alpha chain, Lectin beta chain, CALCIUM ION, ... (5 entities in total)
• Functional Keywords: galactose binding, seed lectin, hemagglutinin, legume lectin, anti fungal, sugar binding protein
• Biological source: Spatholobus parviflorus; More
• Total number of polymer chains: 4
• Total formula weight: 103579.59

Authors

Geethanandan, K.,Bharath, S.R.,Abhilash, J.,Sadasivan, C.,Haridas, M. (deposition date: 2009-08-18, release date: 2009-09-08, Last modification date: 2023-11-01)

Primary citation

Geethanandan, K.,Abhilash, J.,Bharath, S.R.,Sadasivan, C.,Haridas, M.
X-ray structure of a galactose-specific lectin from Spatholobous parviflorous
Int.J.Biol.Macromol., 49:992-998, 2011

PubMed Abstract: A galactose-specific seed lectin from Spatholobous parviflorus (SPL) has been purified, crystallized and its X-ray structure solved. It is the first lectin purified and crystallized from the genus Spatholobus (family: Fabaceae). The crystals belong to the space group P1, with a=60.792 Å, b=60.998 Å, c=78.179 Å, α=78.68°, β=88.62°, γ=104.32°. The data were collected at 2.04 Å resolution under cryocondition, on a MAR image-plate detector system, mounted on a rotating anode X-ray generator. The coordinates of Dolichos biflorus lectin (1lu1) were successfully used for the structure solution by molecular replacement method. The primary structure of the SPL was not known earlier and it was unambiguously visible in the electron density. S. parviflorus lectin is a hetero-dimeric-tetramer with two alpha and two beta chains of 251 and 239 residues respectively. SPL has two metal ions, Ca(2+) and Mn(2+), bound to a loop region of each chain. The SPL monomers are in jelly roll form.

PubMed: 21889532
DOI: 10.1016/j.ijbiomac.2011.08.021

Experimental method

X-RAY DIFFRACTION (2.04 Å)