3IPV
Crystal structure of Spatholobus parviflorus seed lectin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-03-12 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 60.998, 60.792, 78.179 |
| Unit cell angles | 101.32, 91.38, 104.32 |
Refinement procedure
| Resolution | 19.820 - 2.040 |
| R-factor | 0.20197 |
| Rwork | 0.199 |
| R-free | 0.26442 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lu1 |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.952 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP (AUTO MR) |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.000 | 2.140 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.072 | 0.211 |
| Number of reflections | 62376 | |
| <I/σ(I)> | 11.1 | 3.1 |
| Completeness [%] | 90.5 | 73.2 |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 25% PEG 8000, 0.2M Phosphate Buffer Saline, 5% MPD, 5% Iso propanol, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
