3IOI

Crystal structure of the Fucosylgalactoside alpha N-acetylgalactosaminyltransferase (GTA, cisAB mutant L266G, G268A) in complex with a novel UDP-Gal derived inhibitor (1GW)

3IOI の概要

• エントリーDOI: 10.2210/pdb3ioi/pdb
• 関連するPDBエントリー: 1LZ0 3IOH 3IOJ
• 分子名称: Histo-blood group ABO system transferase, MANGANESE (II) ION, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: gta, abo, cisab mutant, aa(gly)b, rossmann fold, inhibitor, semi-closed conformation, blood group antigen, glycoprotein, glycosyltransferase, golgi apparatus, manganese, membrane, metal-binding, polymorphism, secreted, signal-anchor, transferase, transmembrane
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein: P16442
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35481.45

構造登録者

Pesnot, T.,Jorgensen, R.,Palcic, M.M.,Wagner, G.K. (登録日: 2009-08-14, 公開日: 2010-04-07, 最終更新日: 2023-09-06)

主引用文献

Pesnot, T.,Jorgensen, R.,Palcic, M.M.,Wagner, G.K.
Structural and mechanistic basis for a new mode of glycosyltransferase inhibition.
Nat.Chem.Biol., 6:321-323, 2010

PubMed Abstract: Glycosyltransferases are carbohydrate-active enzymes with essential roles in numerous important biological processes. We have developed a new donor analog for galactosyltransferases that locks a representative target enzyme in a catalytically inactive conformation, thus almost completely abolishing sugar transfer. Results with other galactosyltransferases suggest that this unique mode of glycosyltransferase inhibition may also be generally applicable to other members of this important enzyme family.

PubMed: 20364127
DOI: 10.1038/nchembio.343

実験手法

X-RAY DIFFRACTION (1.45 Å)